Anammox Bakterilerinin Zenginleştirilmesinde Farklı Dolgu Malzemelerinin Etkisi / The Effect of Different Filling Materials in Anammox Bacteria Enrichment

Dilek ÖZGÜN, Serden BAŞAK, Kevser CIRIK, ARZU KILIÇ, Dilek Akman, şebnem ÖZDEMİR, ÖZER ÇINAR

Özet


Anaerobik amonyum oksidasyonu (Anammox) anoksik ortamda amonyumun elektron verici nitritin elektron alıcı olarak azot gazına oksitlendiği proses olarak bilinmektedir. Konvansiyonel nitrifikasyon-denitrifikasyon prosesleriyle karşılaştırıldığında Anammox prosesinde daha az oksijen kullanılmakta ve hiçbir organik madde (metanol, glikoz) içeriğine gerek duyulmamaktadır. Ancak sayılan avantajlarının yanı sıra Anammox bakterilerinin yavaş büyüme oranı (11-30 gün) dezavantajını oluşturmaktadır. Dolayısıyla bu bakterilerin zenginleştirilme safhasında özellikle kesikli reaktörler ile çalışmalar yapılmaktadır. Bu çalışmada sürekli olarak işletilen yukarı akışlı reaktörde (UASB-Upflow anaerobic sludge blanket), farklı dolgu malzemeleri kullanılarak hassas ve yavaş büyüyen Anammox bakterilerinin sistemden dışarıya atılmasının engellenmesi amaçlanmaktadır. Sistem yukarı akışlı kolon reaktörde 2 gün hidrolik bekleme süresinde (HRT-Hydraulic retention time) işletilmiştir. Çalışmada seramik taşlar ve Linpor dolgu malzemesi kullanılmıştır. Her iki dolgu malzemesi ile 45 gün işletilen reaktörlerden seramik taşların kullanıldığı reaktörde amonyum azotunun giderimin hızlı bir şekilde %90’lara ulaştığı gözlenmiştir. Linpor dolgu maddelerinin kullanıldığı reaktörde ise amonyum azotunun giderimi daha yavaş olmuştur. Nitrit azotu ise her iki reaktörde de %90’lara varan giderime ulaşmıştır. Stokiyometrik denkleme göre kıyaslandığında Linporlarda çok fazla miktarda nitrat azotunun oluştuğu görülmüştür. 25 gün sonunda Linporlu reaktörde elde edilen sonuçlarla seramik taş dolgulu reaktördeki sonuçlarla benzerlik göstermiştir. The Effect of Different Filling Materials in Anammox Bacteria Enrichment Anaerobic ammonium oxidation (Anammox) is a process that ammonium as electron donor is oxidized to nitrogen gas using nitrite as electron acceptor. Compared to conventional nitrification-denitrification processes, this process is used less oxygen and no organic material (methanol, glucose). However, the slow growth rate of Anammox bacteria (11-30 days) is disadvantages. Therefore, batch reactors have been carried out in these bacteria enrichment. In this study continuously operated upflow anaerobic sludge reactor (UASB) using different filling materials disposing of sensitive and slow-growing Anammox bacteria out of the system is purposed. System is operated up-flow column reactor at 2 days hydraulic retention time (HRT). In this study, ceramic stones and Linpor filling material are used. Both filling material reactors are operated in 45 days. Ceramic stones filling reactor is observed quickly reaches 90% were used reactor ammonium removal. The ammonium nitrogen removal was slower in Linpor filling materials reactor. Nitrite removal is reached up to 90% in both the reactor. When compared to the stoichiometric equation in Linpor was composed of large amounts of nitrate. At the end of 25 days the results were similar to ceramic stone filling reactor with Linpor filling material reactors.

Anahtar Kelimeler


Anammox, Yukarı Akışlı Reaktör, Hidrolik Bekletme Süresi, Seramik Taş, Linpor, Anammox, Upflow Anaerobic Sludge Blanket, Hydraulic Retention Time, Ceramic Stone, Linpor

Tam Metin:

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DOI: http://dx.doi.org/10.7596/taksad.v1i4.80

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